Human E-Cadherin ELISA Kit - Quantikine

Catalog #: DCADE0B
9 Citations Datasheet / COA / SDS

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DCADE0B

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QC295: Quantikine Immunoassay Control 1102 for Human E-Cadherin

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All E-Cadherin ELISAs

Product Details

Citations (9)

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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human E-Cadherin Quantikine ELISA Kit Summary

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL)

Sensitivity

0.126 ng/mL

Assay Range

0.6 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)

Specificity

Natural and recombinant human E-Cadherin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Sample Type	Mean (ng/mL)	Range (ng/mL)
Serum (n=30)	250	127-492
EDTA Plasma (n=30)	242	131-479
Heparin Plasma (n=30)	236	116-479
Urine (n=10)	60.6	3.03-121
Saliva (n=10)	145	21.4-278

Product Summary

The Quantikine™ Human E-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human E-Cadherin in cell culture supernates, serum, plasma, saliva, and urine. It contains HEK293-expressed recombinant human E-Cadherin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human E-Cadherin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human E-Cadherin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.42	7.06	14.6	2.19	6.55	14.2
Standard Deviation	0.047	0.145	0.266	0.136	0.368	0.845
CV%	1.9	2.1	1.8	6.2	5.6	6

Recovery

The recovery of human E-Cadherin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=30)	242	131-479
Heparin Plasma (n=30)	236	116-479
Saliva (n=10)	145	21.4-278
Serum (n=30)	250	127-492
Urine (n=10)	60	3.03-121

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human E-Cadherin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human E-Cadherin ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: E-Cadherin

The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2⁺-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.

Entrez Gene IDs:

999 (Human); 12550 (Mouse); 83502 (Rat)

Alternate Names:

Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

Related Research Areas

Citations for Human E-Cadherin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Doublecortin-like kinase 1 promotes hepatocyte clonogenicity and oncogenic programming via non-canonical &beta-catenin-dependent mechanism
Authors: N Ali, CB Nguyen, P Chandrakes, RF Wolf, D Qu, R May, T Goretsky, J Fazili, TA Barrett, M Li, MM Huycke, MS Bronze, CW Houchen
Sci Rep, 2020-06-29;10(1):10578.
Species: Human
Sample Types: Cell Culture Supernates
Structure establishment of three-dimensional (3D) cell culture printing model for bladder cancer
Authors: MJ Kim, BH Chi, JJ Yoo, YM Ju, YM Whang, IH Chang
PLoS ONE, 2019-10-22;14(10):e0223689.
Species: Human
Sample Types: Cell Culture Supernates
Targeting the temporal dynamics of hypoxia-induced tumor-secreted factors halts tumor migration
Authors: M Singh, XJ Tian, VS Donnenberg, AM Watson, J Zhang, LP Stabile, SC Watkins, J Xing, S Sant
Cancer Res., 2019-04-05;0(0):.
Species: Human, Xenograft
Sample Types: Cell Culture Supernates
Meloxicam executes its antitumor effects against hepatocellular carcinoma in COX-2- dependent and -independent pathways.
Authors: Dong, Xiaofeng, Li, Rui, Xiu, Peng, Dong, Xuesong, Xu, Zongzhen, Zhai, Bo, Liu, Feng, Jiang, Hongchi, Sun, Xueying, Li, Jie, Qiao, Haiquan
PLoS ONE, 2014-03-27;9(3):e92864.
Species: Human
Sample Types: Cell Culture Supernates
Polymorphonuclear neutrophils promote dyshesion of tumor cells and elastase-mediated degradation of E-cadherin in pancreatic tumors.
Authors: Gaida M, Steffen T, Gunther F, Tschaharganeh D, Felix K, Bergmann F, Schirmacher P, Hansch G
Eur J Immunol, 2012-11-02;42(12):3369-80.
Species: Human
Sample Types: Cell Culture Supernates
Serum levels of beta-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma.
Authors: Zekri A, Bahnassy A, Alam El-Din H, Morsy H, Shaarawy S, Moharram N, Daoud S
Oncol Rep, 2011-06-21;26(4):825-31.
Species: Human
Sample Types: Serum
Epithelial adhesion molecules can inhibit HIV-1-specific CD8+ T-cell functions.
Authors: Streeck H, Kwon DS, Pyo A, Flanders M, Chevalier MF, Law K, Julg B, Trocha K, Jolin JS, Anahtar MN, Lian J, Toth I, Brumme Z, Chang JJ, Caron T, Rodig SJ, Milner DA, Piechoka-Trocha A, Kaufmann DE, Walker BD, Altfeld M
Blood, 2011-03-14;117(19):5112-22.
Species: Human
Sample Types: Plasma
Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.
Authors: Hung YJ, Lee CH, Chu NF
Diabetes Care, 2010-05-26;33(8):1840-4.
Species: Human
Sample Types: Plasma
Elevated serum levels of soluble E-cadherin in patients with primary Sjogren's syndrome.
Authors: Jonsson MV, Salomonsson S, Oijordsbakken G, Skarstein K
Scand. J. Immunol., 2005-12-01;62(6):552-9.
Species: Human
Sample Types: Serum